ENCAB000AUW

Bos taurus

any cell type or tissue

characterized to standards with exemption

Homo sapiens

any cell type or tissue

characterized to standards with exemption

thymusthymus

Method: immunoblot

exempt from standards

Caption: Primary antibody characterization Antibody: H3K36me3 Vendor: Abcam Part number: ab9050 Lot number: GR52625-1
Submitter comment: I agree that since there is not similar string of peptides in H4 and that the modified peptide blocks binding to both bands, that the lower band is very likely to be a proteolyzed product of the upper band. I think that this explanation should be included in the figure legend and then this antibody would pass.
Reviewer comment: that seems like a very reasonable suggestion (i.e. including the hypothesis about proteolysis in the validation document). I've attached to this email a new draft validation that contains wording about the hypothesis that the lower molecular weight bands are due to proteolysis. Please consider this as the replacement draft validation for this particular antibody.
Submitted by: Noam Shoresh
Lab: Bradley Bernstein, Broad
Grant: U54HG006991
Download: H3K36me3 Abcam 9050 Lot GR52625-1 western blot v2 (1).pdf
Documents: encode:Histone_Modification_Antibody_Characterization_ENCODE3_Oct2016.pdf

Method: dot blot assay

compliant

Caption: Performed dot blot analysis using a large set of modified peptides. The only peptide recognized by the H3K36me3 antibody was the correct one.
Submitted by: Meital Hatan
Lab: Bradley Bernstein, Broad
Grant: U54HG006991
Download: H3K36me3_abcam_ab9050_lot_GR52625-1_DotBlot.png
Documents: encode:Antibody_Characterization_ENCODE3_February2014.pdf