ENCAB000AUT
Antibody against Homo sapiens H3K27me3
Homo sapiens
any cell type or tissue
characterized to standards with exemption
- Status
- released
- Source (vendor)
- Millipore
- Product ID
- 07-449
- Lot ID
- DAM1662423
- Characterized targets
- H3K27me3 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Aliases
- bradley-bernstein:PchAb 70-V
- External resources
Characterizations
H3K27me3 (Homo sapiens)
Method: ChIP-seq comparison
compliant
- Caption
- This validation relies on the use of antibodies to a histone modification (H3K27me3) in Oci-Ly-7 cells, and the demonstration that highly similar patterns of enrichment are obtained with each antibody. Please note that the two antibodies originate from two lots of the same vendor antibody part number. The first track shown used an antibody to H3K27me3 (PchAb 70-V, ENCAB000AUT), and the second track shown used an antibody to H3K27me3 previously characterized to Encode standards (PchAb 683, ENCAB000BLA).
- Submitted by
- Nina Farrell
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991
- Download
- H3K27me3 PchAb 70-V WIP SAV.pdf
H3K27me3 (Homo sapiens)
K562
Method: immunoblot
exempt from standards
- Submitter comment
- These lots are being used as one at the lab and this experiment has replicate concordance.
- Reviewer comment
- This is the characterization for a different lot: /antibodies/ENCAB000ASB/. These lots were used in the same experiment with IDR concordance.
- Submitted by
- Charles Epstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991