1. Antibodies
  2. Homo sapiens
  3. TFDP1

ENCAB000AUI

Antibody against Homo sapiens TFDP1

Homo sapiens
K562, HepG2, at least one cell type or tissue
not characterized to standards
Status
released
Source (vendor)
GeneTex
Product ID
GTX113275
Lot ID
40422
Characterized targets
TFDP1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Isotype
IgG
Antigen description
Recombinant fgmt within amino acids 120 and 390 of DP1 (Uniprot ID#Q14186)
External resources

Characterizations

TFDP1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment from submitter
Attachment from submitters
not compliant
Caption
Analysis of gel fragment from GM12878: The sample was analyzed on a LTQ XL Linear Ion Trap Mass Spectrometer by LC-ESI-MS/MS. Peptides were identified using SEQUEST tandem mass spectral analysis with probability based matching at p < 0.05. SEQUEST results were reported with ProteinProphet protXML Viewer (TPP v4.4 JETSTREAM) and filtered for a minimum probability of 0.9. All protein hits that met these criteria were reported, including common contaminants. Fold enrichment for each protein reported was determined using a custom script based on the FC-B score calculation from the reference Mellacheruvu et al., 2013. The CRAPome: a contaminant repository for affinity purification mass spectrometry data. Nat. Methods. 10(8):730-736. Doi:10.1038/nmeth.2557. The target protein, TFDP1, was identified as the 57th ranked enriched protein and the 2nd ranked transcription factor based on IP-Mass Spectrometry.
Submitter comment
The standards say 20th or less, this is 57th
Submitted by
Mark Mackiewicz
Lab
Richard Myers, HAIB
TFDP1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not reviewed
Caption
GM12878 whole cell lysate was immunoprecipitated using the primary antibody (GeneTex; GTX113275). The IP fraction was loaded on a 12% Bio-Rad TGX gel and separated with the Bio-Rad Tetra Cell system. A gel fragment (rectangle outline) corresponding to the band indicated on the Coomassie Blue stained gel image was excised and sent to the University of Alabama at Birmingham Cancer Center Mass Spectrometry/Proteomics Shared Facility.
Submitter comment
This image is to show which bands are used for the mass spec
Submitted by
Mark Mackiewicz
Lab
Richard Myers, HAIB
TFDP1 (Homo sapiens)
K562HepG2
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Whole cell lysates of K562 and GM12878 were immunoprecipitated using the primary antibody (GeneTex; GTX113275). The IP fraction was separated on a 12% acrylamide gel with the Bio-Rad PROTEAN II xi system. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. The membrane was probed with the primary antibody (same as that used for IP) and a secondary HRP-conjugated antibody. The resulting bands were visualized with SuperSignal West Femto Solution (Thermo Scientific). Protein Marker (PM) is labeled in kDa. One band was detected at ~42 kDa.
Submitted by
Flo Pauli-Behn
Lab
Richard Myers, HAIB
Download
 TFDP1_IP_WB.png