ENCAB000AUG
Antibody against Homo sapiens FUBP1
Homo sapiens
K562
characterized to standards
- Status
- released
- Source (vendor)
- GeneTex
- Product ID
- GTX115154
- Lot ID
- 40401
- Characterized targets
- FUBP1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Isotype
- IgG
- External resources
Characterizations
FUBP1 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against FUBP1 in HepG2 whole cell lysate using FUBP1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FUBP1.FUBP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- FUBP1-HEPG2.png
FUBP1 (Homo sapiens)
Method: knockdown or knockout
compliant
- Caption
- Western blot following shRNA against FUBP1 in K562 whole cell lysate using FUBP1 specific antibody. Lane 1 is a ladder, lane 2 is k562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FUBP1.FUBP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
- Submitted by
- Xintao Wei
- Lab
- Brenton Graveley, UConn
- Grant
- U54HG007005
- Download
- FUBP1-k562.png
FUBP1 (Homo sapiens)
K562
Method: immunoprecipitation
compliant
- Caption
- IP-Western blot analysis of K562 whole cell lysate using FUBP1 specific antibody. Lane 1 and lane 4 are 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation using FUBP1 antibody, Lane 3 is 50% of IP enrichment using rabbit polyclonal FUBP1 antibody and lane 5 is 50% of IP enrichement using rabbit normal IgG control antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
- Submitted by
- Balaji Sundararaman
- Lab
- Gene Yeo, UCSD
- Grant
- U54HG007005
- Download
- GeneTex_GTX115154_40401_FUBP1.png