ENCAB000AQU

Antibody against Homo sapiens H3K4me1

Homo sapiens
any cell type or tissue
not characterized to standards
Status
released
Source (vendor)
Active Motif
Product ID
39297
Lot ID
21008001
Characterized targets
H3K4me1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
crude
Isotype
serum
Antigen description
This Histone H3 monomethyl Lys4 antibody was raised against a peptide including monomethyl-lysine 4 of human histone H3.
Aliases
bradley-bernstein:PchAb 531
External resources

Characterizations

H3K4me1 (Homo sapiens)
Method: dot blot assay
not compliant
Caption
Performed dot blot analysis using a large set of modified peptides. The peptide most strongly recognized by the H3K4me1 antibody was the correct one.
Submitter comment
There is not a 10-fold enriched binding signal for the modification of interest relative to other modifications.
Submitted by
Kathrina Onate
Lab
Bradley Bernstein, Broad
H3K4me1 (Homo sapiens)
H1-hESCHepG2K562
Method: immunoblot
compliant
Caption
0.7ug unmodified Recombinant Histone H3 (Abcam ab1791) and nuclear extract from 10ug HepG2 and K562 cells were resolved by electrophoresis on a 4-12% acrylamide gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4 C. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Panel A: anti Histone H3 (Abcam ab1791), band of expected size (~17kDa) visualized in both unmodified recombinant histone H3 and NL, at similar intensities. Panel B: Band of expected size (~17kDa) visualized in HepG2 and K562 nuclear lysates, and cannot be detected in unmodified recombinant Histone H3.
Submitted by
Kathrina Onate
Lab
Bradley Bernstein, Broad