ENCAB000ANO

Antibody against Homo sapiens H3K9ac

Homo sapiens
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Millipore
Product ID
07-352
Lot ID
unknown
Characterized targets
H3K9ac (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
crude

Characterizations

H3K9ac (Homo sapiens)
Method: ChIP-seq comparison
not reviewed
Caption
ChIP-seq analysis shows that the H3K9Ac antibody pulls down the same fragments as does the H3K4me3 antibody in both K562 and Ntera2 cells.
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford
H3K9ac (Homo sapiens)
Method: immunoblot
not reviewed
Caption
Western Blot Analysis Representative lot data. Acid extracts from untreated (lane 1) and sodium butyrate treated (lane 2) HeLa cells (Catalog # 17-305) were resolved by electrophoresis, transferred to nitrocellulose and probed with antiacetyl- Histone H3 (Lys9), (1:5000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates acetyl-Histone H3 (Lys9), (~17 kDa).
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford