ENCAB000ALQ
Antibody against Homo sapiens TBL1XR1
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Novus
- Product ID
- NB600-270
- Lot ID
- 001
- Characterized targets
- TBL1XR1 (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Immunogen: A synthetic peptide that maps to a region between residues 100 and 150 of human TBL1-related Protein 1 using the numbering given in SwissProt entry Q9BZK7.
- External resources
Characterizations
TBL1XR1 (Homo sapiens)
Method: immunoprecipitation
not reviewed
- Caption
- Because the overlap of the top 40% of peaks from datasets derived from antibodies NB600-270 and ab24550 (compared using standard ENCODE scoring methods) exceeds the recommended threshold of 80%, both antibodies meet this criterion for validation.
- Submitted by
- Michael Snyder
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558
TBL1XR1 (Homo sapiens)
Method: ChIP-seq comparison
not reviewed
- Caption
- Using antibody ab24550 or NB600-270, we observe a single band in Western blots on lysates from cell lines K562 and HeLa S3, that migrates at a position consistent with the predicted size (~56kD) of TBLR1 (Panels A,C). This band is specifically and efficiently immunoprecipitated from K562 lysates by each antibody (Panels B,D). Based on this, ab24550 and NB600-270 are validated by this criterion.
- Submitted by
- Michael Snyder
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558