ENCAB000AJL
Antibody against Homo sapiens WHSC1
Homo sapiens
HeLa
partially characterized
Homo sapiens
K562, HepG2, GM12878, endothelial cell of umbilical vein
not characterized to standards
- Status
- released
- Source (vendor)
- Abcam
- Product ID
- ab75359
- Lot ID
- GR47579-1
- Characterized targets
- WHSC1 (Homo sapiens)
- Host
- mouse
- Clonality
- monoclonal
- Purification
- affinity
- Antigen description
- Raised against a fusion protein, corresponding to amino acids 1-647 of Human GST-WHSC1/NSD2.
- Aliases
- bradley-bernstein:PchAb 291
- External resources
Characterizations
WHSC1 (Homo sapiens)
HeLa
compliant
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570
WHSC1 (Homo sapiens)
not reviewed
- Caption
- Antibody was validated by use of correlation analysis of ChIP-String data obtained using this antibody together with diverse other antibodies
- Submitted by
- Bradley Bernstein
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG004570
WHSC1 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
not compliant
- Caption
- Nuclear lysates from GM12878 (10ug),HUVEC (10ug), HepG2 (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. The strongest band detected is of the expected size (~80kDa). **(Molecular mass: 152258 Da)**
- Reviewer comment
- Multiple bands and marked band not at least 50% of total signal. 80 kDa corresponds to isoform 2, but isoform 1 = 152 kDa... should add a second arrow @ the 152 band?
- Submitted by
- Noam Shoresh
- Lab
- Bradley Bernstein, Broad
- Grant
- U54HG006991