ENCAB000AJL

Antibody against Homo sapiens WHSC1

Homo sapiens
HeLa
partially characterized
Homo sapiens
K562, HepG2, GM12878, endothelial cell of umbilical vein
not characterized to standards
Status
released
Source (vendor)
Abcam
Product ID
ab75359
Lot ID
GR47579-1
Characterized targets
WHSC1 (Homo sapiens)
Host
mouse
Clonality
monoclonal
Purification
affinity
Antigen description
Raised against a fusion protein, corresponding to amino acids 1-647 of Human GST-WHSC1/NSD2.
Aliases
bradley-bernstein:PchAb 291

Characterizations

WHSC1 (Homo sapiens)
HeLa
Method: immunoblot
compliant
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
WHSC1 (Homo sapiens)
Method: ChIP-string comparison
not reviewed
Caption
Antibody was validated by use of correlation analysis of ChIP-String data obtained using this antibody together with diverse other antibodies
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
WHSC1 (Homo sapiens)
K562HepG2GM12878endothelial cell of umbilical vein
Method: immunoblot
Attachment from submitter
not compliant
Caption
Nuclear lysates from GM12878 (10ug),HUVEC (10ug), HepG2 (10ug), were loaded into a 4-12% Bis-Tris gel in 1X MES running buffer. After separation, the samples were transferred to a nitrocellulose membrane using iblot. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. The strongest band detected is of the expected size (~80kDa). **(Molecular mass: 152258 Da)**
Reviewer comment
Multiple bands and marked band not at least 50% of total signal. 80 kDa corresponds to isoform 2, but isoform 1 = 152 kDa... should add a second arrow @ the 152 band?
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad