ENCAB000AJC
Antibody against Homo sapiens NFYA
Homo sapiens
at least one cell type or tissue
awaiting characterization
- Status
- released
- Source (vendor)
- Roberto Mantovani
- Product ID
- NF-YA
- Lot ID
- unknown
- Characterized targets
- NFYA (Homo sapiens)
- Host
- rabbit
- Clonality
- polyclonal
- Purification
- affinity
- Antigen description
- Raised against amino acids 139-347 of recombinant human NFYA.
- External resources
Characterizations
NFYA (Homo sapiens)
not reviewed
- Caption
- HeLaS3 nuclear extract (5x10^7cells/IP) was incubated with 10ug of anti-NFYA or normal rabbit IgG (SC-2027, Santa Cruz Biotechnology) and immunoprecipitated with protein A sepharose beads. Input nuclear extract, supernatant after IP and elution fractions were analysed by Western blotting as above, except: 10-14% gradient gel, 1:700 dilution anti-NFYA. Specific immunoprecipitation of the short and long NFYA isoforms was observed only in the anti-NFYA IP (indicated by arrows). Additional validation was carried out by observing specific ChIP-QPCR signals at known NFY bound genomic loci, and by comparison to NFYB ChIP-Seq data showing high degree of correlation between NFYA and NFYB read counts at NFY bound regions across 3 cell lines (Pearson correlation >0.7).
- Submitted by
- Kevin Struhl
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558
- Download
- human_NF-YA_validation_Struhl.pdf
NFYA (Homo sapiens)
not reviewed
- Caption
- Nuclear extract, of the indicated cell lines, were separated on a 10% gel using SDS-PAGE, transferred to a PDF membrane and Western blotted using standard protocols. Primary antibody (anti-NFYA, gift from Prof. R. Mantovani) and secondary antibody (SC-2317, Santa Cruz Biotechnology) were used at a dilution of 1:2500 and 1:4000, respectively. Signal was detected using Luminol Reagent (SC-2048, Santa Cruz Biotechnology). Detection of the known short and long NFYA isoforms are indicated by arrows.
- Submitted by
- Kevin Struhl
- Lab
- Michael Snyder, Stanford
- Grant
- U54HG004558
- Download
- human_NF-YA_validation_Struhl.pdf