ENCAB000AHY

Antibody against Homo sapiens IRF3

Homo sapiens
K562, SK-N-SH, HepG2, GM12878
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Status
released
Source (vendor)
Santa Cruz Biotech
Product ID
sc-9082
Lot ID
I0908
Characterized targets
IRF3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Antigen description
Epitope corresponding to amino acids 1-425 of IRF-3 of human origin.

Characterizations

IRF3 (Homo sapiens)
Method: immunoblot
not reviewed
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
IRF3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Arrow indicates immunoprecipitated band of expected size for IRF3 from K562 nuclear lysates. Lane 1 = HeLa-S3 nuclear lysate. Lane 2 = K562 nuclear lysate. Lane 3 = supernatant from K562 immunoprecipitation (IP) Lane 4 = bound material from K562 IP Lane 5 = bound material from control IgG IP from K562
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
IRF3 (Homo sapiens)
SK-N-SH
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: SK-N-SH using the antibody sc-9082. The image shows western blot analysis of input, flowthrough, immunoprecipitate, and mock immunoprecipitate using IgG. Target molecular weight: 47KD.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
IRF3 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
compliant
Caption
IP followed by mass spectrometry: Briefly, protein was immunoprecipitated from K562 whole cell lysates using sc-9082, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGE Bis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was silver-stained, gel fragments corresponding to the bands indicated were excised and destained using the SilverSNAP Stain for Mass Spectrometry (Pierce). Then proteins were treated with trypsin using the in-gel digestion method. Digested proteins were analyzed on a LTQ-Orbitrap (Thermo Scientific) b the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (protein false discovery rate <1%, 2 peptides per protein minimum).
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
IRF3 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
compliant
Caption
Immunoprecipitation of IRF3 from K562 cells using sc-9082. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with sc-9082. Lane 3: material immunoprecipitated using control IgG. Band A was excised from gel and subject to analysis by mass spectrometry. Expected band size ~47kD.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
Download
IRF3 (Homo sapiens)
HepG2GM12878K562
Method: immunoblot
Attachment from submitter
compliant
Caption
Arrows indicate band consistent with expected size (47kD) of IRF3 in LEFT PANEL: whole cell lysates from HepG2 cells and RIGHT PANEL: whole cell lysates of GM12878 and K562 cells (from left to right). Although there are two images for the immunoblot, we are considering them as one image. We consider HepG2 as lane 1, GM12878 as lane 2, and K562 as lane 3.
Reviewer comment
Although there are two images for the immunoblot, we are considering them as one image. We consider HepG2 as lane 1, GM12878 as lane 2, and K562 as lane 3.
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford