1. Antibodies
  2. Homo sapiens
  3. IKZF1

ENCAB000AHV

Antibody against Homo sapiens IKZF1

Homo sapiens
at least one cell type or tissue
awaiting characterization
Status
released
Source (vendor)
Stephen Smale
Product ID
IKZF1_(IkN)
Lot ID
unknown
Characterized targets
IKZF1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
crude
Antigen description
Raised against a recombinant protein encompassing the amino-terminus of the murine IKZF1 protein.

Characterizations

IKZF1 (Homo sapiens)
Method: ChIP-seq comparison
Attachment PDF Icon
not reviewed
Caption
ChIP-seq Peak overlap comparison of two different anti-Ikaros antibodies. Independent ChIP-seq assays were performed in GM12878 cells using two different Ikaros antibodies. Peaks were called using the Solesearch peak calling program and the lists were compared. Shown is a 92% agreement of ChIP-seq peaks between the two datasets.
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford
IKZF1 (Homo sapiens)
Method: immunoblot
Attachment PDF Icon
not reviewed
Caption
Western blot analysis of IKZF1 in ENCODE cell nuclear extracts. Nuclear extracts were prepared and 25 ug from the indicated cell-lines were analyzed by SDS-PAGE and Western blotting using the anti-Ikaros antibody at a dilution of 1:1000. As indicated in the blot, a single band corresponding to the correct predicted molecular weight of IKZF1 is detected in most of the extracts. Several splice isoforms for IKZF1 have been reported in both B- and T-cells (http://www.ncbi.nlm.nih.gov/pubmed/7935426).
Submitted by
Peggy Farnham
Lab
Michael Snyder, Stanford