1. Antibodies
  2. Homo sapiens
  3. GTF2F1

ENCAB000AHE

Antibody against Homo sapiens GTF2F1

Homo sapiens
K562, HeLa-S3, HepG2, MCF-7
characterized to standards
Homo sapiens
any cell type or tissue
partially characterized
Homo sapiens
GM12878
not characterized to standards
Status
released
Source (vendor)
Abcam
Product ID
ab28179
Lot ID
GR60531
Characterized targets
GTF2F1 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Antigen description
Recombinant full length protein - RAP 74 subunit of TF11F.

Characterizations

GTF2F1 (Homo sapiens)
HepG2
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: HepG2, using the antibody ab28179. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: 58 kDa.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
GTF2F1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line K562 using the antibody ab28179. Lane 1: input nuclear lysate. Lane 2: material immunoprecipitated with antibody. Lane 3: material immunoprecipitated using control IgG. Marked bands were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 58.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
Download
 1009_GTF2F1.jpg
GTF2F1 (Homo sapiens)
MCF-7
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: MCF-7, using the antibody ab28179. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.
Reviewer comment
The expected size on the image and the target is wrong. This antibody is supposed to target the ~70kD subunit of the TFIIF heterodimer.
Submitted by
Denis Salins
Lab
Michael Snyder, Stanford
GTF2F1 (Homo sapiens)
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
not submitted for review by lab
Caption
Immunoprecipitation was performed on nuclear extracts from the cell line: GM12878, using the antibody ab28179. The blot shows western blot analysis of input, flowthrough, immunoprecipitate and mock immunoprecipitate using IgG.Molecular Weight: None
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
GTF2F1 (Homo sapiens)
Method: immunoprecipitation followed by mass spectrometry
Attachment PDF Icon
compliant
Caption
IP followed by mass spectrometry. Briefly, protein was immunoprecipitated from K562 nuclear cell lysates using the antibody ab28179, and the IP fraction was loaded on a 10% polyacrylamide gel (NuPAGEBis-Tris Gel) and separated with an Invitrogen NuPAGE electrophoresis system. The gel was stained by ColloidialCoomassie G-250 stain, gel fragments corresponding to the bands indicated were excised. Then proteins were trypsinized using the in-gel digestion method. Digested proteins were analyzed on an Orbitrap Elite mass spectrometer (Thermo Scientific) by the nanoLC-ESI-MS/MS technique. Peptides were identified by the SEQUEST algorithm and filtered with a high confidence threshold (Peptide false discovery rate < 1%, 2 unique peptides per protein minimum, mass error < 10 ppm).
Submitter comment
Biogrid: GTF2F1 has interaction with TAT (4 publication), MATR3 also has interaction with TAT (1 publication).http://thebiogrid.org/109217/summary/homo-sapiens/gtf2f1.html, http://thebiogrid.org/115126/summary/homo-sapiens/matr3.html. MATR3 is the the same gel slice with GTF2F1, but TAT is not. Biogrid: GTF2F1 has interaction with PARP1 (2 publication), XRCC5 also has interaction with PARP1 (9 publication).http://thebiogrid.org/109217/summary/homo-sapiens/gtf2f1.html; http://thebiogrid.org/113353/summary/homo-sapiens/xrcc5.html. XRCC5 is the same gel slice with GTF2F1, but PARP1 is not.
Submitted by
Nathaniel Watson
Lab
Michael Snyder, Stanford
GTF2F1 (Homo sapiens)
Method: immunoblot
Attachment PDF Icon
not reviewed
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
GTF2F1 (Homo sapiens)
Method: immunoprecipitation
Attachment PDF Icon
not reviewed
Caption
Arrow indicates band consistent with expected size (predicted size of 58kD but reported to run at ~74kD ) of GTF2F1 in nuclear lysates from (left to right): GM12878, K562, HeLaS3, and HepG2 cell lines. 100kD 75kD 50kd Arrows indicate immunoprecipitated (IP) band of expected size from HeLaS3 nuclear lysates. Lane 1= HeLaS3 nuclear lysate, Lane2 = HeLaS3 whole cell lysate, Lane 3= flow through and Lane4= bound material from HeLaS3 IP with ab28179, Lane 5= bound material from control IgG IP from HeLaS3.
Submitted by
Michael Snyder
Lab
Michael Snyder, Stanford
GTF2F1 (Homo sapiens)
HeLa-S3
Method: immunoprecipitation
Attachment from submitter
Attachment from submitters
compliant
Caption
Arrows indicate immunoprecipitated (IP) band of expected size from HeLaS3 nuclear lysates. Lane 1= HeLaS3 nuclear lysate, Lane2 = HeLaS3 whole cell lysate, Lane 3= flow through and Lane4= bound material from HeLaS3 IP with ab28179, Lane 5= bound material from control IgG IP from HeLaS3.
Reviewer comment
HepG2 compliant
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford
GTF2F1 (Homo sapiens)
GM12878K562HeLa-S3HepG2
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
Arrow indicates band consistent with expected size (predicted size of 58kD but reported to run at ~74kD ) of GTF2F1 in nuclear lysates from (left to right): GM12878, K562, HeLaS3, and HepG2 cell lines. 100kD 75kD 50kd Arrows indicate immunoprecipitated (IP) band of expected size from HeLaS3 nuclear lysates. Lane 1= HeLaS3 nuclear lysate, Lane2 = HeLaS3 whole cell lysate, Lane 3= flow through and Lane4= bound material from HeLaS3 IP with ab28179, Lane 5= bound material from control IgG IP from HeLaS3.
Reviewer comment
1, 3, 4 not compliant; 2 compliant
Submitted by
Kathrina Onate
Lab
Michael Snyder, Stanford