ENCAB000AFE

Antibody against Homo sapiens CHD1

Homo sapiens
at least one cell type or tissue
awaiting characterization
Homo sapiens
GM12878, SK-N-SH, K562, HepG2
partially characterized
Status
released
Source (vendor)
Bethyl Labs
Product ID
A301-218A
Lot ID
1
Host
rabbit
Clonality
polyclonal
Purification
affinity
Aliases
bradley-bernstein:PchAb 49-V

Characterizations

CHD1 (Homo sapiens)
K562
Method: immunoprecipitation
Attachment from submitter
not compliant
Caption
K562 whole cell lysate was immunoprecipitated using primary antibody, and the IP fraction along side a whole cell lysate were loaded on a CriterionXT gel and separated. After separation, the samples were transferred using a wet transfer. Blotting with primary (same as that used for IP) and secondary HRP-conjugated antibodies was performed. Band of expected size visualized representing strongest signal in the lane.
Reviewer comment
No IgG control. UniProt size is 196 KD and gel notes say to expect 220 KD.
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
CHD1 (Homo sapiens)
K562HepG2GM12878SK-N-SH
Method: immunoblot
Attachment from submitter
compliant
Caption
Nuclear lysates from K562 (10ug), HepG2 (7ug), GM12878 (7ug), SK-N-SH (6ug), were loaded into a 4-12% Bis-Tris gel in 1X MOPS running buffer. After separation, the samples were transferred to a PVDF membrane using a wet transfer. Membrane was blocked for an hour in room temperature, with 5% BSA in TBS-T and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. a single band of the expected size was detected (~240kDa).
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
CHD1 (Homo sapiens)
Method: immunoblot
not reviewed
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad
CHD1 (Homo sapiens)
Method: ChIP-string comparison
not reviewed
Caption
Antibody was validated by use of correlation analysis of ChIP-String and ChIP-Seq data
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad