Mus musculus
any cell type or tissue
characterized to standards with exemption
Status
released
Source (vendor)
Abcam
Product ID
ab8895
Lot ID
720417
Characterized targets
H3K4me1 (Mus musculus)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3, mono methylated at K4
Aliases
bing-ren:5.4

Characterizations

H3K4me1 (Mus musculus)
ES-Bruce4
Method: immunoblot
Attachment from submitter
exempt from standards
Caption
An immunoblot using whole cell extract of a mouse ES cell line shows a positive band at the size of the histone (17kDa) but also shows a faint band around 37kDa. The histone band detected in the extract constitutes 96.63% of the protein signal and shows at least 10-fold enrichment relative to any other band.
Submitter comment
There is not enough antibody of this lot to recreate the Western up to the ENCODE3 standards. We have other lots of this same antibody that have the immunoblot up to the newer quality standards: ENCAB027VOE and ENCAB969VGQ
Reviewer comment
As per conversations on the Binding Group call, we accept this antibody validation with exemption from standards document.
Submitted by
David Gorkin
Lab
Bing Ren, UCSD
H3K4me1 (Mus musculus)
Method: dot blot assay
Attachment from submitter
compliant
Caption
Performed dot blot analysis using a large set of modified peptides.The only peptide recognized by the H3K4me1 antibody was the correct one (noted with #1).
Submitted by
Ah Young Lee
Lab
Bing Ren, UCSD