1. Antibodies
  2. Homo sapiens
  3. HIST2H3A + HIST2H3D + HIST2H3C

ENCAB000ADU

Antibody against Homo sapiens H3K36me3

Homo sapiens
any cell type or tissue
characterized to standards
Status
released
Source (vendor)
Abcam
Product ID
ab9050
Lot ID
499302
Characterized targets
H3K36me3 (Homo sapiens)
Host
rabbit
Clonality
polyclonal
Purification
affinity
Isotype
IgG
Antigen description
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3, tri methylated at K36.
Aliases
bradley-bernstein:PchAb 78-V

Characterizations

H3K36me3 (Homo sapiens)
K562HEK293
Method: immunoblot
Attachment from submitter
Attachment from submitters
compliant
Caption
0.25ug unmodified Recombinant Histone H3 (Millipore 14-494) and 30ug of K562 Whole Cell Lysate (Abcam, ab7911) were resolved by electrophoresis on a 4-12% acrylamide gel. After separation, the samples were transferred to a nitrocellulose membrane with an Invitrogen iBlot system. Membrane was blocked for an hour in room temperature, with 5% nonfat dry milk and blotted with primary antibody in the appropriate concentration over night at 4c. Membrane was washed and blotted with secondary HRP-conjugated antibody. Detection was made with Optiblot ECL Detect Kit (ab133406) for 2 min. Panel A: anti Histone H3 (Abcam ab1791), band of expected size (~17kDa) visualized in both unmodified recombinant histone H3 and WCL, at similar intensities.Panel B: Band of expected size (~17kDa) visualized in WCL, and cannot be detected in unmodified recombinant Histone H3. Antibody seems to be specific since no reactivity with nonhistone proteins or unmodified histones was detected
Submitted by
Noam Shoresh
Lab
Bradley Bernstein, Broad
H3K36me3 (Homo sapiens)
Method: dot blot assay
Attachment PDF Icon
compliant
Caption
Suitably modified peptides modeled on histone tails were obtained from Abcam. Each peptide is added to Bio-Dot apparatus at 0.01 and 0.1 ug / 50uL total volume (BioRad) Let sit on rocker 60 minutes. Pull through with vacuum, wash once with 100uL TBS and twice with 200uL TBSTw. Remove membrane from Bio-Dot and transfer to 10mL Pierce SuperBlock in tray. Place on rocker for 30 minutes. Dump off SuperBlock.
Submitted by
Bradley Bernstein
Lab
Bradley Bernstein, Broad