Experiment summary for ENCSR992WXP
doi:10.17989/ENCSR992WXP
Summary
- Status
- released
- Assay
- ChIP-seq (Histone ChIP-seq)
- Target
- H3K27me3
- Biosample summary
- Homo sapiens BLaER1 6 hours after the sample was treated with 100 nM 17β-estradiol, 10 ng/mL Interleukin-3, 10 ng/mL CSF1
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- H3K27me3 ChIP-Seq on BLaER1 cell line after 6 h treatment with 100 nM 17β-estradiol, 10 ng/mL Interleukin-3, 10 ng/mL CSF1.
- Treatments
- 100 nM 17β-estradiol (CHEBI:16469) , 10 ng/mL Interleukin-3 (UniProtKB:P08700) , 10 ng/mL CSF1 (UniProtKB:P09603)
- Nucleic acid type
- DNA
- Average fragment size
- 432 [2-1]367 [1-1]
- Fragmentation methods
- shearing (Covaris generic)
- Size selection method
- AMPure XP bead purification
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
Community submission
- Lab
- Roderic Guigo, CRG
- Award
- ERC-2011-AdG-294653-RNA-MAPS (Roderic Guigo, CRG)
- Project
- community
- External resources
- Aliases
- roderic-guigo:td_chipseq_H006H3K27me3X_experiment
- Date released
- May 10, 2022
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Antibody | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens BLaER1 cell line 6 hours after the sample was treated with 100 nM 17β-estradiol, 10 ng/mL Interleukin-3, 10 ng/mL CSF1 | ENCBS411JBC | ENCAB830MOW | ENCLB312ROH |
| 1 | 1 | Homo sapiens BLaER1 cell line 6 hours after the sample was treated with 100 nM 17β-estradiol, 10 ng/mL Interleukin-3, 10 ng/mL CSF1 | ENCBS584ALJ | ENCAB830MOW | ENCLB299TBE |