Experiment summary for ENCSR940IMN
doi:10.17989/ENCSR940IMN
Summary
- Status
- released
- Assay
- RNA-seq (total RNA-seq)
- Biosample summary
- Mus musculus strain C57BL/6J macrophage chromatin fraction
- Biosample Type
- primary cell
- Replication type
- isogenic
- Description
- Ribo depletion RNA-seq on chromatin fraction RNA which is part of BMDM cell, stimulated with 100 ng/ml Lipid A for 10 minute.
- Nucleic acid type
- RNA
- Depleted in
- rRNA
- Library starting quantity
- 1µg [1-1]500ng [3-1]250ng [2-1]
- Size range
- 250-400
- Fragmentation methods
- see document
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Platform
- Illumina HiSeq 4000, Illumina HiSeq 2000
Attribution
Genomics of Gene Regulation Project
- Lab
- Alexander Hoffmann, UCLA
- Award
- U01HG007912 (Alexander Hoffmann, UCLA)
- Project
- GGR
- External resources
- Aliases
- alexander-hoffmann:chromatin.Naive.010.exp
- Date submitted
- November 30, 2017
- Date released
- November 20, 2018
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Mus musculus strain C57BL/6J macrophage primary cell chromatin fraction | ENCBS472UHG | ENCLB237UQO |
| 3 | 1 | Mus musculus strain C57BL/6J macrophage primary cell chromatin fraction | ENCBS710HYS | ENCLB129LBU |
| 2 | 1 | Mus musculus strain C57BL/6J macrophage primary cell chromatin fraction | ENCBS109JNO | ENCLB157TTZ |