Experiment summary for ENCSR660WMS
doi:10.17989/ENCSR660WMS
Summary
- Status
- released
- Assay
- ChIP-seq (Histone ChIP-seq)
- Target
- H4K20me1
- Biosample summary
- Homo sapiens BLaER1 48 hours after the sample was treated with 100 nM 17β-estradiol, 10 ng/mL Interleukin-3, 10 ng/mL CSF1
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- H4K20me1 ChIP-Seq on BLaER1 cell line after 48 h treatment with 100 nM 17β-estradiol, 10 ng/mL Interleukin-3, 10 ng/mL CSF1.
- Treatments
- 100 nM 17β-estradiol (CHEBI:16469) , 10 ng/mL Interleukin-3 (UniProtKB:P08700) , 10 ng/mL CSF1 (UniProtKB:P09603)
- Nucleic acid type
- DNA
- Average fragment size
- 684 [2-1]559 [1-1]
- Fragmentation methods
- shearing (Covaris generic)
- Size selection method
- AMPure XP bead purification
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
Community submission
- Lab
- Roderic Guigo, CRG
- Award
- ERC-2011-AdG-294653-RNA-MAPS (Roderic Guigo, CRG)
- Project
- community
- External resources
- Aliases
- roderic-guigo:td_chipseq_H048H4K20me1X_experiment
- Date released
- May 10, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Antibody | Library |
|---|---|---|---|---|---|
| 2 | 1 | Homo sapiens BLaER1 cell line 48 hours after the sample was treated with 100 nM 17β-estradiol, 10 ng/mL Interleukin-3, 10 ng/mL CSF1 | ENCBS766HUL | ENCAB786WVD | ENCLB655AKK |
| 1 | 1 | Homo sapiens BLaER1 cell line 48 hours after the sample was treated with 100 nM 17β-estradiol, 10 ng/mL Interleukin-3, 10 ng/mL CSF1 | ENCBS777PXO | ENCAB786WVD | ENCLB332HUD |