ENCBS400CEG / cell line

Status: released
Term name: MCF-7
Term ID: EFO:0001203
Summary: Homo sapiens MCF-7 cell line genetically modified (insertion) using CRISPR targeting H. sapiens MSX2
Culture harvest date: 2018-09-14
Separated from biosample: ENCBS370OCG

Accession	Category	Purpose	Method	Nucleic acid delivery method	Site
ENCGM388GHO	insertion	tagging	CRISPR		Target MSX2-human

Caption excerpt: Immunoprecipitation was performed on nuclear extracts from biosample…

Caption: Immunoprecipitation was performed on nuclear extracts from biosample ENCBS400CEG (MCF-7 eGFP-MSX2 replicate 2) cells using anti-eGFP antibody. The image shows western blot analysis of input (lane 1), immunoprecipitate (lane 2), and mock immunoprecipitate using IgG (lane 3). Molecular weight standard (Bio-Rad, cat. # 161-0374) contains 10 pre-stained recombinant proteins (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kD). The target molecular weight is 57.0 kD as indicated with an arrow.
Submitted by: Nathaniel Watson
Lab: Michael Snyder, Stanford
Grant: UM1HG009442
Documents: michael-snyder:E4_P3_IP-WB_v2

Description: Cell growth protocol for MCF-7 cells (Snyder lab, version 1).

Description excerpt: MCF-7 transfection protocol - Version 2 (Replicates split right after…

Description: MCF-7 transfection protocol - Version 2 (Replicates split right after transfection, 600 µg/ml G418)
Submitted by: Nathaniel Watson
Lab: Michael Snyder, Stanford
Grant: UM1HG009442