ENCBS370OCG / cell line
Summary
- Status
- released
- Term name
- MCF-7
- Term ID
- EFO:0001203
- Summary
- Homo sapiens MCF-7 cell line genetically modified (insertion) using CRISPR targeting H. sapiens MSX2
- Separated from biosample
- ENCBS333DBP
- Parent of biosamples
- ENCBS400CEG
Attribution
ENCODE4 project
- Lab
- Michael Snyder, Stanford
- Award PI
- Michael Snyder, Stanford
- Submitted by
- Nathaniel Watson
- Source
- Michael Snyder
- Project
- ENCODE
- External resources
- Aliases
- michael-snyder:pB-11124, michael-snyder:pB-eGFP-MSX2 MCF-7 (batch 3)
Genetic modifications
Accession | Category | Purpose | Method | Nucleic acid delivery method | Site |
|---|---|---|---|---|---|
| ENCGM388GHO | insertion | tagging | CRISPR |
|
Donor information
- Status
- released
- Accession
- ENCDO000AAE
- Aliases
- encode:donor of MCF-7, bradley-bernstein:Donor of MCF-7 cells
- Species
- Homo sapiens
- Life stage
- adult
- Age
- 69 years
- Sex
- female
- Health status
- breast cancer (adenocarcinoma)
- Ethnicity
- European
- External resources
- References
Documents
growth protocol
Description: Cell growth protocol for MCF-7 cells (Snyder lab, version 1).
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442
transfection protocol
Description excerpt: MCF-7 transfection protocol - Version 2 (Replicates split right after…
- Description
- MCF-7 transfection protocol - Version 2 (Replicates split right after transfection, 600 µg/ml G418)
- Submitted by
- Nathaniel Watson
- Lab
- Michael Snyder, Stanford
- Grant
- UM1HG009442