SPOT (Signal Portion of Tags)

Measures signal-to-noise in genome-wide epigenetic profiling assays by calculating the fraction of reads that fall in tag-enriched regions (see the Hotspot program) from a sample of 5 million reads. The SPOT methodology can be generalized to use any peak-finder. A publication of SPOT and a more complete description are in preparation. SPOT is simply the percentage of all tags that fall in hotspots, and the publication for the Hotspot quality metric is found at PMID: 21258342.
Software type
quality metric
Used for
data QC