Showing 9 of 9 results
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- proliferation CRISPR screen in DNA cloning sampleFunctional characterization series for tiling CRIPSRi proliferation screens at genome-wide cCREs essential for neuronal differentiationAssays: proliferation CRISPR screenLab: Yin Shen, UCSFProject: ENCODEElements selection method: accessible genome regions, histone modifications, essential genes
- STARR-seq in glutamatergic neuronHomo sapiens glutamatergic neuron, 7 days post differentiation genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2Lab: Kevin White, UChicagoProject: ENCODE
- STARR-seq in glutamatergic neuronHomo sapiens glutamatergic neuron, 14 days post differentiation genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2 originated from WTC11Lab: Kevin White, UChicagoProject: ENCODE
- CRISPR cutting FACS screen of SIN3A locus in glutamatergic neuron with endogenous protein Sort-seq readout of SIN3AHomo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, (insertion) using transduction, using CRISPR cutting (pgRNA) for SIN3A locus
- CRISPR cutting FACS screen of APP locus in glutamatergic neuron with endogenous protein Sort-seq readout of APPHomo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, (insertion) using transduction, using CRISPR cutting (pgRNA) for APP locus
- CRISPR cutting FACS screen of SIN3A locus in glutamatergic neuron with endogenous protein Sort-seq readout of SIN3AHomo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, (insertion) using transduction, using CRISPR cutting (pgRNA) for SIN3A locus
- CRISPR cutting FACS screen of MECP2 locus in glutamatergic neuron with endogenous protein Sort-seq readout of MECP2Homo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens MECP2, (insertion) using transduction, using CRISPR cutting (pgRNA) for MECP2 locus
- CRISPR cutting FACS screen of FMR1 locus in glutamatergic neuron with endogenous protein Sort-seq readout of FMR1glutamatergic neuron in vitro differentiated cells genetically modified (insertion) using transduction, genetically modified (insertion) using CRISPR targeting H. sapiens FMR1, genetically modified (CRISPR cutting) using CRISPR for FMR1 locus,
- CRISPR cutting FACS screen of APP locus in glutamatergic neuron with endogenous protein Sort-seq readout of APPHomo sapiens glutamatergic neuron genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, (insertion) using transduction, using CRISPR cutting (pgRNA) for APP locus