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Showing 14 of 14 results

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2550100200
  • MPRA in WTC11
    Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test neuronal enhancers and promoters.
    Lab: Nadav Ahituv, UCSF
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR548AQS
    released
  • CRISPRi screen in WTC11
    WTC11 cell line genetically modified (insertion) using transduction, genetically modified (CRISPRi) using CRISPR
    Assays: CRISPR screen
    Lab: Bing Ren, UCSD
    Project: ENCODE
    Series
    ENCSR499VAT
    released
  • proliferation CRISPR screen in WTC11
    Functional characterization series for tiling CRIPSRi proliferation screens at genome-wide cCREs essential for neuronal differentiation
    Assays: proliferation CRISPR screen
    Lab: Yin Shen, UCSF
    Project: ENCODE
    Elements selection method: accessible genome regions, histone modifications, essential genes
    Series
    ENCSR737MRW
    released
  • MPRA in WTC11
    Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.
    Lab: Nadav Ahituv, UCSF
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR336MKI
    released
  • MPRA in WTC11
    Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential WTC11 enhancers and promoters of protein-coding genes.
    Lab: Nadav Ahituv, UCSF
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR244FWB
    released
  • CRISPRi proliferation screen of multiple loci in WTC11
    Homo sapiens WTC11 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple loci
    Assays: proliferation CRISPR screen
    Lab: Yin Shen, UCSF
    Project: ENCODE
    Elements selection method: accessible genome regions
    Tiling modality: peak tiling
    Series
    ENCSR534RBC
    released
  • CRISPR cutting proliferation screen of multiple loci in WTC11
    Homo sapiens WTC11 genetically modified (insertion) using transduction, using CRISPR cutting (sgRNA) for multiple loci
    Assays: proliferation CRISPR screen
    Lab: Yin Shen, UCSF
    Project: ENCODE
    Elements selection method: accessible genome regions
    Tiling modality: peak tiling
    Series
    ENCSR499GLI
    released
  • STARR-seq in WTC11
    Homo sapiens WTC11 genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2
    Lab: Kevin White, UChicago
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR749AQU
    released
  • CRISPR cutting FACS screen of SIN3A locus in WTC11 with endogenous protein Sort-seq readout of SIN3A
    Homo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, (insertion) using transduction, using CRISPR cutting (pgRNA) for SIN3A locus
    Assays: FACS CRISPR screen
    Lab: Yin Shen, UCSF
    Project: ENCODE
    Loci: SIN3A
    Tiling modality: full tiling
    Series
    ENCSR066VPH
    released
  • CRISPR cutting FACS screen of APP locus in WTC11 with endogenous protein Sort-seq readout of APP
    Homo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, (insertion) using transduction, using CRISPR cutting (pgRNA) for APP locus
    Assays: FACS CRISPR screen
    Lab: Yin Shen, UCSF
    Project: ENCODE
    Loci: APP
    Tiling modality: full tiling
    Series
    ENCSR875WZX
    released
  • CRISPR cutting FACS screen of APP locus in WTC11 with endogenous protein Sort-seq readout of APP
    Homo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, (insertion) using transduction, using CRISPR cutting (pgRNA) for APP locus
    Assays: FACS CRISPR screen
    Lab: Yin Shen, UCSF
    Project: ENCODE
    Loci: APP
    Tiling modality: full tiling
    Series
    ENCSR759NRS
    released
  • CRISPR cutting FACS screen of FMR1 locus in WTC11 with endogenous protein Sort-seq readout of FMR1
    WTC11 cell line genetically modified (CRISPR cutting) using CRISPR, genetically modified (insertion) using transduction, genetically modified (insertion) using CRISPR targeting H. sapiens FMR1 , for FMR1 locus
    Assays: FACS CRISPR screen
    Lab: Yin Shen, UCSF
    Project: ENCODE
    Loci: FMR1
    Tiling modality: full tiling
    Series
    ENCSR783EED
    released
  • CRISPR cutting FACS screen of SIN3A locus in WTC11 with endogenous protein Sort-seq readout of SIN3A
    Homo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, (insertion) using transduction, using CRISPR cutting (pgRNA) for SIN3A locus
    Assays: FACS CRISPR screen
    Lab: Yin Shen, UCSF
    Project: ENCODE
    Loci: SIN3A
    Tiling modality: full tiling
    Series
    ENCSR192UAI
    released
  • CRISPR cutting FACS screen of MECP2 locus in WTC11 with endogenous protein Sort-seq readout of MECP2
    Homo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens MECP2, (insertion) using transduction, using CRISPR cutting (pgRNA) for MECP2 locus
    Assays: FACS CRISPR screen
    Lab: Yin Shen, UCSF
    Project: ENCODE
    Loci: MECP2
    Tiling modality: full tiling
    Series
    ENCSR855IIN
    released