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- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.Elements selection method: synthetic elementsLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT enhancer, using 3' to 5' direction.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT1 enhancer.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT1 enhancer.
- MPRA of LDLR locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the LDLR promoter.
- MPRA of LDLR locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the LDLR promoter.
- MPRA of F9 locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the F9 promoter.
- MPRA in DNA cloning sampleThese MPRA datasets test a mix of short, medium, and long elements cloned into the pGL4 vector upstream of a minimal promoter.Assays: MPRALab: Nadav Ahituv, UCSFProject: ENCODEElements selection method: DNase hypersensitive sites
- MPRA in DNA cloning sampleThese MPRA datasets test the same set of elements cloned into the pGL4 vector upstream of a minimal promoter in both the forward and reverse orientations in parallel.Assays: MPRALab: Nadav Ahituv, UCSFProject: ENCODEElements selection method: DNase hypersensitive sites
- MPRA in DNA cloning sampleThese MPRA datasets screen a library of 2,440 candidate liver enhancers and controls for regulatory activity in HepG2 cells using nine different MPRA designs.Assays: MPRALab: Nadav Ahituv, UCSFProject: ENCODEElements selection method: DNase hypersensitive sites
- CRISPRi proliferation screen of multiple loci in HepG2Homo sapiens HepG2 genetically modified (insertion) using transduction, using CRISPRi (pgRNA) for multiple loci
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: DNase hypersensitive sites, histone modifications, synthetic elementsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: DNase hypersensitive sites, histone modifications, synthetic elementsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE