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  • MPRA in WTC11
    Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test neuronal enhancers and promoters.
    Lab: Nadav Ahituv, UCSF
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR548AQS
    released
  • CRISPRi Flow-FISH screen in K562 with PrimeFlow readout of MYC
    Homo sapiens K562 genetically modified (CRISPRi) using CRISPR
    Lab: Jesse Engreitz, Stanford
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR443VTK
    released
  • CRISPRi FACS screen in activated CD4-positive, CD25-positive, alpha-beta regulatory T cell with fluorescence activated cell sorting readout of Tnfrsf18
    Mus musculus strain Rosa26-LSL-dCas9-KRAB activated CD4-positive, CD25-positive, alpha-beta regulatory T cell genetically modified (CRISPRi) using CRISPR
    Elements selection method: accessible genome regions
    Tiling modality: peak tiling
    Lab: Tim Reddy, Duke
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR202QXN
    released
  • CRISPRi screen in WTC11
    WTC11 cell line genetically modified (insertion) using transduction, genetically modified (CRISPRi) using CRISPR
    Assays: CRISPR screen
    Lab: Bing Ren, UCSD
    Project: ENCODE
    Series
    ENCSR499VAT
    released
  • proliferation CRISPR screen in WTC11
    Functional characterization series for tiling CRIPSRi proliferation screens at genome-wide cCREs essential for neuronal differentiation
    Assays: proliferation CRISPR screen
    Lab: Yin Shen, UCSF
    Project: ENCODE
    Elements selection method: accessible genome regions, histone modifications, essential genes
    Series
    ENCSR737MRW
    released
  • MPRA in SK-N-SH
    Homo sapiens SK-N-SH, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfection
    Lab: Ryan Tewhey, JAX
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR959NUX
    released
  • MPRA in HepG2
    Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfection
    Lab: Ryan Tewhey, JAX
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR167HAR
    released
  • MPRA in K562
    Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfection
    Lab: Ryan Tewhey, JAX
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR341GVP
    released
  • MPRA in HepG2
    Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfection
    Lab: Ryan Tewhey, JAX
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR694VUM
    released
  • MPRA in K562
    Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfection
    Lab: Ryan Tewhey, JAX
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR909JVP
    released
  • MPRA in K562
    Homo sapiens K562, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfection
    Lab: Ryan Tewhey, JAX
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR820RPI
    released
  • MPRA in WTC11
    Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.
    Lab: Nadav Ahituv, UCSF
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR336MKI
    released
  • MPRA in HepG2
    Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.
    Lab: Nadav Ahituv, UCSF
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR405QCT
    released
  • MPRA in K562
    Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.
    Lab: Nadav Ahituv, UCSF
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR203UFY
    released
  • MPRA in WTC11
    Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential WTC11 enhancers and promoters of protein-coding genes.
    Lab: Nadav Ahituv, UCSF
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR244FWB
    released
  • STARR-seq in K562
    Homo sapiens K562 genetically modified (episome) using transient transfection
    Lab: Tim Reddy, Duke
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR926NDZ
    released
  • STARR-seq in glutamatergic neuron
    Homo sapiens glutamatergic neuron, 7 days post differentiation genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2
    Lab: Kevin White, UChicago
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR258QGF
    released
  • perturbation followed by snATAC-seq in K562
    K562 cell line genetically modified (insertion) using transduction, genetically modified (CRISPRi) using CRISPR
    Assays: perturbation followed by snATAC-seq
    Lab: Will Greenleaf, Stanford
    Project: ENCODE
    Series
    ENCSR513XAG
    released
  • CRISPR cutting screen of GATA1 locus in K562
    Homo sapiens K562 genetically modified (insertion) using transduction (low MOI), using CRISPR cutting (sgRNA) for GATA1 locus
    Assays: CRISPR screen
    Lab: Yin Shen, UCSF
    Project: ENCODE
    Loci: GATA1
    Tiling modality: peak tiling
    Series
    ENCSR825HCS
    released
  • MPRA in HepG2
    Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.
    Elements selection method: synthetic elements
    Lab: Nadav Ahituv, UCSF
    Project: ENCODE
    Functional Characterization Experiment
    ENCSR632EPR
    released
  • enhancer reporter assay in whole organism
    strain FVB/NCrl whole organism embryo (11.5 days) genetically modified (insertion) using mouse pronuclear microinjection
    Lab: Len Pennacchio, LBNL
    Project: ENCODE
    Transgenic Enhancer Experiment
    ENCSR164QHU
    released
  • enhancer reporter assay in whole organism
    strain FVB/NCrl whole organism embryo (11.5 days) genetically modified (insertion) using mouse pronuclear microinjection
    Lab: Len Pennacchio, LBNL
    Project: ENCODE
    Transgenic Enhancer Experiment
    ENCSR492UFM
    released
  • enhancer reporter assay in whole organism
    strain FVB/NCrl whole organism embryo (11.5 days) genetically modified (insertion) using mouse pronuclear microinjection
    Lab: Len Pennacchio, LBNL
    Project: ENCODE
    Transgenic Enhancer Experiment
    ENCSR036ESM
    released
  • enhancer reporter assay in whole organism
    strain FVB/NCrl whole organism embryo (11.5 days) genetically modified (insertion) using mouse pronuclear microinjection
    Lab: Len Pennacchio, LBNL
    Project: ENCODE
    Transgenic Enhancer Experiment
    ENCSR806RFP
    released
  • enhancer reporter assay in whole organism
    strain FVB/NCrl whole organism embryo (11.5 days) genetically modified (insertion) using mouse pronuclear microinjection
    Lab: Len Pennacchio, LBNL
    Project: ENCODE
    Transgenic Enhancer Experiment
    ENCSR781BZX
    released