Showing 21 of 21 results
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- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test neuronal enhancers and promoters.Lab: Nadav Ahituv, UCSFProject: ENCODE
- CRISPRi CRISPR screen in WTC11Homo sapiens WTC11, 14 days post-nucleic acid delivery time genetically modified (insertion) using transduction, using CRISPRi (pgRNA)Lab: Bing Ren, UCSDProject: ENCODE
- proliferation CRISPR screen in WTC11Homo sapiens WTC11, 0 days post differentiation genetically modified (CRISPRi) using CRISPR, (insertion) using CRISPR, (insertion) using TALEN inserting M. musculus Neurog2Lab: Yin Shen, UCSFProject: ENCODE
- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in WTC11Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential WTC11 enhancers and promoters of protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA of SHH locus in NIH3T3Elements cloned into the pGL4Z vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZRS enhancer.
- MPRA of SHH locus in NIH3T3Elements cloned into the pGL4Z vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the ZRS enhancer.
- MPRA of IRF6 locus in HaCaTElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the IRF6 enhancer.
- STARR-seq in WTC11Homo sapiens WTC11 genetically modified (episome) using transient transfection, (insertion) using TALEN inserting M. musculus Neurog2Lab: Kevin White, UChicagoProject: ENCODE
- CRISPR cutting proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPR cutting proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPR cutting proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPRi proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPRi proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPRi proliferation screen of multiple loci in WTC11Cell survival-based CRISPR screen targeting genomic regions near MSH2, MSH6, MLH1, PMS2, PCNA and HPRT1 genes.
- CRISPR cutting FACS screen of FMR1 locus in WTC11 with endogenous protein Sort-seq readout of FMR1Homo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens FMR1, (insertion) using transduction, using CRISPR cutting (pgRNA) for FMR1 locus
- CRISPR cutting FACS screen of APP locus in WTC11 with endogenous protein Sort-seq readout of APPHomo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, (insertion) using transduction, using CRISPR cutting (pgRNA) for APP locus
- CRISPR cutting FACS screen of SIN3A locus in WTC11 with endogenous protein Sort-seq readout of SIN3AHomo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, (insertion) using transduction, using CRISPR cutting (pgRNA) for SIN3A locus
- CRISPR cutting FACS screen of MECP2 locus in WTC11 with endogenous protein Sort-seq readout of MECP2Homo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens MECP2, (insertion) using transduction, using CRISPR cutting (pgRNA) for MECP2 locus
- CRISPR cutting FACS screen of APP locus in WTC11 with endogenous protein Sort-seq readout of APPHomo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens APP, (insertion) using transduction, using CRISPR cutting (pgRNA) for APP locus
- CRISPR cutting FACS screen of SIN3A locus in WTC11 with endogenous protein Sort-seq readout of SIN3AHomo sapiens WTC11 genetically modified (insertion) using CRISPR (pgRNA) targeting H. sapiens SIN3A, (insertion) using transduction, using CRISPR cutting (pgRNA) for SIN3A locus