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- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test potential HepG2, K562, and WTC11 enhancers and promoters of all protein-coding genes.Lab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.Elements selection method: synthetic elementsLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA of TERT locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the TERT promoter.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT enhancer, using 3' to 5' direction.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT1 enhancer.
- MPRA of SORT1 locus in HepG2Elements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the SORT1 enhancer.
- MPRA of MYC locus in HEK293TElements cloned into the pGL4.23 vector upstream of a minimal promoter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the MYC enhancer around SNP rs6983267.
- MPRA of MSMB locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the MSMB promoter.
- MPRA of LDLR locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the LDLR promoter.
- MPRA of LDLR locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the LDLR promoter.
- MPRA of HNF4A locus in HEK293TElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the HNF4A promoter.
- MPRA of FOXE1 locus in HeLaElements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the FOXE1 promoter.
- MPRA of F9 locus in HepG2Elements cloned into the pGL4.11 vector upstream of the reporter, with barcode located in the 3' UTR of the reporter. Elementas are generated by error-prone PCR of the F9 promoter.
- MPRA in HCT116Homo sapiens HCT116, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HCT116Homo sapiens HCT116, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2, 24 hours post-nucleic acid delivery time genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in mammary epithelial cellHomo sapiens mammary epithelial cell female genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HepG2Homo sapiens HepG2 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HEK293Homo sapiens HEK293 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- MPRA in HEK293Homo sapiens HEK293 genetically modified (episome) using transient transfectionElements selection method: sequence variantsLab: Ryan Tewhey, JAXProject: ENCODE
- perturbation followed by snATAC-seq in MCF-7CRISPRi perturbation of MCF7sLab: Will Greenleaf, StanfordProject: ENCODE
- MPRA in HepG2Elements cloned into the lentiMPRA vector upstream of a minimal promoter, with barcode located in the 5' UTR of the reporter. Elements test HepG2 enhancers and promoters of all protein-coding genes.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the pGL4 vector upstream of a minimal promoter. Test of a mix of short, medium, and long elements in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE
- MPRA in HepG2Elements cloned into the pGL4 vector upstream of a minimal promoter. Test of the same set of elements in both the forward and reverse orientations in parallel.Elements selection method: DNase hypersensitive sitesLab: Nadav Ahituv, UCSFProject: ENCODE