Ruan Lab ChIA-PIPE Pipeline

Status
released
Title
Ruan Lab ChIA-PIPE Pipeline
Assays
ChIA-PET
Description
ChIA-PIPE is a fully automated pipeline for ChIA-PET data processing, quality assessment, visualization, and analysis. ChIA-PIPE performs linker filtering, read mapping, peak calling, and loop calling and automates quality control assessment for each dataset. To enable visualization, ChIA-PIPE generates input files for two-dimensional contact map viewing with Juicebox and HiGlass and provides a new dockerized visualization tool for high-resolution, browser-based exploration of peaks and loops. To enable structural interpretation, ChIA-PIPE calls chromatin contact domains, resolves allele-specific peaks and loops, and annotates enhancer-promoter loops. ChIA-PIPE also supports the analysis of other related chromatin-mapping data types.
Lab
Yijun Ruan, JAX
Award PI
Yijun Ruan, JAX

Pipeline schematic

Documents

pipeline protocol
Description: Ruan Lab ChIA-PIPE processing pipeline for ChIA-PET data
Submitted by
Bonita Lam
Lab
Yijun Ruan, JAX