ENCGM708ABH

Summary

Status
released
Description
wgIs202 [ceh-9::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov, M, et al. Nat Methods (2006) 10:839-44. Gerstein MB, et al. Science. 2010 Dec 24;330(6012):1775-87. Strain was constructed as part of the Regulatory Element Project, part of modENCODE MADE BY:modEncode RECEIVED:09-04-12 from Kim S, Stanford University Medical School, Stanford, CA
Type
insertion
Tags
  • eGFPC-terminal
  • 3xFLAGC-terminal
Purpose
tagging

Modification site

Coordinates
ce10 chrII:3651773-3691729
Integration site
random

Modification method

Nucleic acid delivery method
bombardment