- This construct contains an eGFP N-terminal tag to GLI2 under control of a CMV promoter, introduced to expression vector pcDNA5/FRT as part of Thermo Fisher's Flp-In system. It is stably integrated at the introduced FLP recombinase target (FRT) site in the host genome.
- eGFP — N-terminal
- Integration site
- site-specific recombination
- Site-specific recombinase Flp (UniProtKB:P03870)