Summary
- Description
- This construct contains an eGFP N-terminal tag to ZSCAN5CP under control of a CMV promoter, introduced to expression vector pcDNA5/FRT as part of Thermo Fisher's Flp-In system. It is stably integrated at the introduced FLP recombinase target (FRT) site in the host genome.
- Type
- insertion
- Introduced sequence
- ATGGCTGCAAATTGCACATCCTCATGGAGTCTAGGAGAATCCTGCAACAGCCCTGGGTCAGAGCCACCACAGTCCATGCCATCCCCAGCAACTCAACTTGGAAATCATGACAGTGATCCTGAGACTTGTCACGTGAACTTCAGGATGTTCAGCTGCCCGAAGGAGTCGGACCCCATCCAGGCTCTGAGGAAACTCACTGAGCTGTGCCATCTGTGGCTGAGGCCCGACCTCCACACCAAAGAGCAGATCCTGGACATGCTGGTGATGGAGCAGTTCATGATCTCCATGCCCCAGGAGCTCCAGGTCTTAGTCATGATGAACGGTGTGCAGAGCTGCAAAGACCTGGAGGACCTGCTACGAAATAACAGAAGACCCAAGAAATGGTCTGTAGTCAGTTTTCTTGGCAAGGAATATCTTATGCAGGAATCAGATGTGGAGATGGCTGAAGCCCCCGCCAGTGTCAGAGATGATCCGAGACACGTGTCCAGCCAGCGGACCTCCTCTGTGAACCAGATGTGTCCGGAGGAAGGCCAGGCCAGCCAAGAGCTGCAGACCCTGCCCAGGGTCCCTGCACTGTTCAGGAGGCAGGAAGAGGACTTCCTGCTGCCAGAGACTACTGTCATGAAAGGTGACCCAAAGGCTCTGAGACCCAAGCCGACCTTGGAGAAGGACCTGGAGGAAGACAGGGAGGAGAACCCAGGACTTACATCCCCAGAGCCTCAGCTTCCAAACAGTCCCACAGGGGTGGTGGGAGCAAAGGAGGGGAAGGAACCCCAAAAAAGAGCCTCTGTGGAAAATGTGGATGCTGACACACCTTCTGCCTGCGTTGTGGAGAGAGAAGCTTCGACTCACAGCGGGAGCAGAGGAGACGCTCTGAATCTGAGAGGTCTCAAAAGAAGCAAACCAGACGCCACCTCCATTTCCCAAGAAGAGCCTCAAGGAGAAGCCACACCTGTGGGCAACAGAGAATCCCCGGGACAAGCTGAGATCAATCCAGTTCATTCCCCAGGCCCTGCGGGCCCAGTCAGTCACCCCAGTGGCCAAGAAGTCAAGGAACTGCTGCCCTTTGCATGTGAGGTGTGCGGCAAGAGGTTTAAGTATCGCGGCAAGTTAGCCGTCCACACGAGATCACACACAGGCGAGAGACTCTTTCAGTGTAATCTCTGCGGGAAGCGCTTCATGCAGCGCATAGGCCTGCAATTTCACCAGCGAACCCACACTGGCGAGAGGCCCTACACGTGTGACATCTGCCAGAAGCAGTTCACCCAGAAGTCCTACTTGAAGTGTCACAAGAGAAGCCACACAGGGGAGAAGCCCTTCGAATGTAAAGACTGCAAGAAAGTTTTCACCTACAAGGCAAATCTGAAGGAGCACCAGCGCATCCACTCTGGAGAGAAACCCCACAAATGTTCCAAGTGTCCAAGAGCCTTCGGTCGGCCGGCGACCTTAAGACGCCACCAGAAAACACATCGAGAAGCCACTTCACAG
- Purpose
- tagging
Modification method
- Method
- site-specific recombination
- Treatments
- Site-specific recombinase Flp (UniProtKB:P03870)