Summary
- Description
- This strain's transgene was constructed by fosmid recombineering at the TransgeneOmics Unit of the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden. The tagged fosmid was integrated into the genome of an unc-119(ed3) worms using biolistic transformation. The UNC-62::EGFP fusion protein is expressed in the correct unc-62 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the UNC-62 transcription factor.
- Type
- insertion
- Tags
- eGFP — C-terminal
- FLAG — C-terminal
- Purpose
- tagging
Modification method
- Nucleic acid delivery method
- bombardment