Functional Characterization Experiment summary for ENCSR202QXN
Summary
- Status
- released
- Assay
- FACS CRISPR screen
- Biosample summary
- Mus musculus strain Rosa26-LSL-dCas9-KRAB activated CD4-positive, CD25-positive, alpha-beta regulatory T cell genetically modified (CRISPRi) using CRISPR
- Biosample Type
- primary cell
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- CRISPR screen readout
- fluorescence activated cell sorting
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Platform
- Illumina MiSeq
- Elements references
Attribution
ENCODE4 project
- Lab
- Tim Reddy, Duke
- Award
- UM1HG009428 (Tim Reddy, Duke)
- Project
- ENCODE
- Aliases
- tim-reddy:mm10-treg-crispri-GITR
- Date released
- August 2, 2024
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Mus musculus strain Rosa26-LSL-dCas9-KRAB activated CD4-positive, CD25-positive, alpha-beta regulatory T primary cell genetically modified (CRISPRi) using CRISPR | ENCBS287HYT | ENCGM589QCU | ENCLB586AZX |
| 2 | 1 | Mus musculus strain Rosa26-LSL-dCas9-KRAB activated CD4-positive, CD25-positive, alpha-beta regulatory T primary cell genetically modified (CRISPRi) using CRISPR | ENCBS028PPA | ENCGM589QCU | ENCLB508ATJ |
| 3 | 1 | Mus musculus strain Rosa26-LSL-dCas9-KRAB activated CD4-positive, CD25-positive, alpha-beta regulatory T primary cell genetically modified (CRISPRi) using CRISPR | ENCBS710GOT | ENCGM589QCU | ENCLB965HON |
| 4 | 1 | Mus musculus strain Rosa26-LSL-dCas9-KRAB activated CD4-positive, CD25-positive, alpha-beta regulatory T primary cell genetically modified (CRISPRi) using CRISPR | ENCBS350FCZ | ENCGM589QCU | ENCLB268NDZ |
| 2 | 2 | Mus musculus strain Rosa26-LSL-dCas9-KRAB activated CD4-positive, CD25-positive, alpha-beta regulatory T primary cell genetically modified (CRISPRi) using CRISPR | ENCBS028PPA | ENCGM589QCU | ENCLB480DXI |
| 1 | 2 | Mus musculus strain Rosa26-LSL-dCas9-KRAB activated CD4-positive, CD25-positive, alpha-beta regulatory T primary cell genetically modified (CRISPRi) using CRISPR | ENCBS287HYT | ENCGM589QCU | ENCLB173ZGU |
| 3 | 2 | Mus musculus strain Rosa26-LSL-dCas9-KRAB activated CD4-positive, CD25-positive, alpha-beta regulatory T primary cell genetically modified (CRISPRi) using CRISPR | ENCBS710GOT | ENCGM589QCU | ENCLB395RIN |
| 4 | 2 | Mus musculus strain Rosa26-LSL-dCas9-KRAB activated CD4-positive, CD25-positive, alpha-beta regulatory T primary cell genetically modified (CRISPRi) using CRISPR | ENCBS350FCZ | ENCGM589QCU | ENCLB216EYY |