Functional Characterization Experiment summary for ENCSR034NTW
doi:10.17989/ENCSR034NTW
Summary
- Status
- released
- Assay
- Flow-FISH CRISPR screen
- Biosample summary
- Homo sapiens K562 genetically modified (insertion) using transduction, using CRISPRi (sgRNA) for multiple loci
- Biosample Type
- cell line
- Perturbation type
- CRISPRi
- Tiling modality
- peak tiling
- Examined loci
- SEC61A1 (10-20%)
- CRISPR screen readout
- PrimeFlow
- Replication type
- isogenic
- Nucleic acid type
- DNA
- Size range
- 200-210
- Platform
- Illumina HiSeq 4000
Attribution
ENCODE4 project
- Lab
- Jesse Engreitz, Stanford
- Award
- UM1HG009436 (Will Greenleaf, Stanford)
- Project
- ENCODE
- Aliases
- jesse-engreitz:CRISPRiFlowFISH-K562-SEC61A1-binB_funccharexp
- Date released
- January 7, 2021
- Functional Characterization Series
- ENCSR760TSA
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS464LHY | ENCGM924EDC, ENCGM912CXW | ENCLB961OGK |
| 2 | 1 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS733URR | ENCGM924EDC, ENCGM912CXW | ENCLB835NYZ |
| 1 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS464LHY | ENCGM924EDC, ENCGM912CXW | ENCLB674EMM |
| 1 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS464LHY | ENCGM924EDC, ENCGM912CXW | ENCLB062HMU |
| 2 | 2 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS733URR | ENCGM924EDC, ENCGM912CXW | ENCLB776HDR |
| 1 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS464LHY | ENCGM924EDC, ENCGM912CXW | ENCLB089WXS |
| 2 | 3 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS733URR | ENCGM924EDC, ENCGM912CXW | ENCLB372LGK |
| 2 | 4 | Homo sapiens K562 cell line genetically modified (insertion) using transduction, genetically modified using CRISPRi (sgRNA) | ENCBS733URR | ENCGM924EDC, ENCGM912CXW | ENCLB711XFU |