Experiment summary for ENCSR991HIA
doi:10.17989/ENCSR991HIA
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- hif-1
- Biosample summary
- Caenorhabditis elegans strain OR3349 whole organism L4 larva 52 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans hif-1
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing HIF-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:HIF-1-OR3349-L4
- Date submitted
- October 5, 2017
- Date released
- November 6, 2017
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 2 | 1 | Caenorhabditis elegans strain OR3349 mixed sex L4 larva 52 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans hif-1 | ENCBS754SFL | ENCGM330VHI | ENCAB728YTO | ENCLB522TOW |
| 1 | 1 | Caenorhabditis elegans strain OR3349 mixed sex L4 larva 52 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans hif-1 | ENCBS990NDG | ENCGM330VHI | ENCAB728YTO | ENCLB808EXW |