Experiment summary for ENCSR980XNE
doi:10.17989/ENCSR980XNE
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- npax-4
- Biosample summary
- Caenorhabditis elegans strain OP493 (unc-119(tm4063) III; wgIs493) whole organism hermaphrodite late embryo 4 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans npax-4
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing NPAX-4-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:NPAX-4-OP493-LE
- Date submitted
- July 29, 2015
- Date released
- May 10, 2016
- Submitter comment
- ENCFF515TZH, ENCFF645POC, ENCFF062SMA, ENCFF115XCQ were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
1 | 1 | Caenorhabditis elegans strain OP493 (unc-119(tm4063) III; wgIs493) hermaphrodite late embryo 4 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans npax-4 | ENCBS386VJJ | ENCGM416HQF | ENCAB728YTO | ENCLB429ZJS |
2 | 1 | Caenorhabditis elegans strain OP493 (unc-119(tm4063) III; wgIs493) hermaphrodite late embryo 4 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans npax-4 | ENCBS800ZFC | ENCGM416HQF | ENCAB728YTO | ENCLB820OIU |