Experiment summary for ENCSR978WED
doi:10.17989/ENCSR978WED
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- jim
- Biosample summary
- Drosophila melanogaster strain jim-GFP whole organism wandering third instar larva genetically modified (insertion) using site-specific recombination targeting D. melanogaster jim
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic flies expressing jim-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 175-275 [1-1]200-325 [3-1]
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- E-Gel
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:jim-GFP
- Date submitted
- July 23, 2015
- Date released
- July 6, 2016
- Submitter comment
- ENCFF348QIX, ENCFF420JYS were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Drosophila melanogaster strain jim-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster jim | ENCBS399HYB | ENCGM389QPB | ENCAB728YTO | ENCLB045PNW |
| 3 | 1 | Drosophila melanogaster strain jim-GFP mixed sex wandering third instar larva whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster jim | ENCBS313CHY | ENCGM389QPB | ENCAB728YTO | ENCLB144GQF |