Experiment summary for ENCSR966JPL
doi:10.17989/ENCSR966JPL
Summary
- Status
- released
- Assay
- polyA plus RNA-seq
- Biosample summary
- Mus musculus strain C57BL/6J spleen tissue male adult (10 weeks)
- Biosample Type
- tissue
- Replication type
- unreplicated
- Description
- RNA-seq of mouse spleen tissue. The second technical replicate was performed in May 2015 and was not part of the original Lin et al. 2014 publication (PMID:25413365).
- Nucleic acid type
- polyadenylated mRNA
- Size range
- 350-450
- Extraction method
- RNeasy Lipid Tissue Mini Kit (QIAGEN cat#74804)
- Fragmentation methods
- chemical (Illumina TruSeq)
- Strand specificity
- Strand-specific (reverse)
- Platform
- Illumina HiSeq 2000, Illumina HiSeq 2500
Attribution
ENCODE Mouse Project
- Lab
- Michael Snyder, Stanford
- Award
- RC2HG005602 (Michael Snyder, Stanford)
- Project
- ENCODE
- External resources
- References
- Date submitted
- January 8, 2015
- Date released
- January 8, 2015
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Mus musculus strain C57BL/6J male adult (10 weeks) spleen tissue | ENCBS614NNG | ENCLB601GXX |
| 1 | 2 | Mus musculus strain C57BL/6J male adult (10 weeks) spleen tissue | ENCBS614NNG | ENCLB726TJR |