Experiment summary for ENCSR964GKZ
doi:10.17989/ENCSR964GKZ
Summary
- Status
- released
- Assay
- polyA plus RNA-seq
- Biosample summary
- Homo sapiens A549 treated with 100 nM dexamethasone (agonist) for 15 minutes
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- mRNA-seq on A549 cell line treated with 100 nM dexamethasone for 15 mins.
- Treatments
- 100 nM dexamethasone (CHEBI:41879) for 15 minutes
- Nucleic acid type
- polyadenylated mRNA
- Library starting quantity
- 1µg
- Size range
- >200
- Extraction method
- RNeasy Plus Mini Kit Qiagen cat#74134 plus additional on column Dnase treatment
- Fragmentation methods
- chemical (RNase III)
- Size selection method
- dual SPRI
- Strand specificity
- Strand-specific (reverse)
- Platform
- Illumina HiSeq 2500
Attribution
Genomics of Gene Regulation Project
- Lab
- Tim Reddy, Duke
- Award
- U01HG007900 (Tim Reddy, Duke)
- Project
- GGR
- External resources
- Aliases
- tim-reddy:t15m_rna_seq
- Date submitted
- October 13, 2017
- Date released
- October 19, 2017
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 2 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 15 minutes | ENCBS013HGJ | ENCLB234SUZ |
| 1 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 15 minutes | ENCBS645JZI | ENCLB363UBQ |
| 3 | 1 | Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 15 minutes | ENCBS326TJA | ENCLB940JYF |