Experiment summary for ENCSR964GKZ

Summary

Status
released
Assay
RNA-seq (polyA RNA-seq)
Biosample summary
Homo sapiens A549 treated with 100 nM dexamethasone for 15 minutes
Biosample Type
cell line
Replication type
isogenic
Description
mRNA-seq on A549 cell line treated with 100 nM dexamethasone for 15 mins.
Treatments
100 nM dexamethasone (CHEBI:41879) for 15 minute
Nucleic acid type
polyadenylated mRNA
Library starting quantity
1µg
Size range
>200
Extraction method
RNeasy Plus Mini Kit Qiagen cat#74134 plus additional on column Dnase treatment
Fragmentation method
chemical (RNase III)
Size selection method
dual SPRI
Strand specificity
Strand-specific

Attribution

Genomics of Gene Regulation Project
Lab
Tim Reddy, Duke
Award
U01HG007900 (Tim Reddy, Duke)
Project
GGR
Aliases
tim-reddy:t15m_rna_seq
Date submitted
October 13, 2017
Date released
October 19, 2017

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleLibrary
21A549 cell line treated with 100 nM dexamethasone for 15 minutesENCBS013HGJENCLB234SUZ
11A549 cell line treated with 100 nM dexamethasone for 15 minutesENCBS645JZIENCLB363UBQ
31A549 cell line treated with 100 nM dexamethasone for 15 minutesENCBS326TJAENCLB940JYF