Experiment summary for ENCSR961TSV

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Drosophila melanogaster strain CG5245-GFP whole organism embryo (0-16 hours) genetically modified using site-specific recombination
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing CG5245-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:CG5245-GFP
Date submitted
April 9, 2016
Date released
July 6, 2016
Submitter comment
ENCFF471QIU, ENCFF755CHN were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
11strain CG5245-GFP mixed sex embryo (0-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-CG5245ENCBS265GHWENCGM552OBWENCAB728YTOENCLB349BNI
31strain CG5245-GFP mixed sex embryo (0-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-CG5245ENCBS208AFEENCGM552OBWENCAB728YTOENCLB079SYM
21strain CG5245-GFP mixed sex embryo (0-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-CG5245ENCBS321ZNWENCGM552OBWENCAB728YTOENCLB744VYK