Experiment summary for ENCSR957OLT
doi:10.17989/ENCSR957OLT
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- mec-3
- Biosample summary
- Caenorhabditis elegans strain OP55 (unc-119(ed3) III; wgIs55) whole organism hermaphrodite late embryo 4 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans mec-3
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing MEC-3-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:MEC-3-OP55-LE
- Date submitted
- July 29, 2015
- Date released
- May 10, 2016
- Submitter comment
- ENCFF579IRS, ENCFF636ZQM, ENCFF285RJI, ENCFF274WYC were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
1 | 1 | Caenorhabditis elegans strain OP55 (unc-119(ed3) III; wgIs55) hermaphrodite late embryo 4 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans mec-3 | ENCBS442IMT | ENCGM779KZJ | ENCAB728YTO | ENCLB095WIQ |
2 | 1 | Caenorhabditis elegans strain OP55 (unc-119(ed3) III; wgIs55) hermaphrodite late embryo 4 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans mec-3 | ENCBS681JZW | ENCGM779KZJ | ENCAB728YTO | ENCLB835TKR |