Experiment summary for ENCSR951SLK
doi:10.17989/ENCSR951SLK
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- ceh-27
- Biosample summary
- Caenorhabditis elegans strain OP139 (wgIs139(ceh-27::TY1 EGFP 3xFLAG(P000007_F10); unc-119(+));unc-119(ed3)) whole organism young adult (73 hours) at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans ceh-27
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing ceh-27-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:CEH-27_OP139_yAd
- Date submitted
- May 30, 2018
- Date released
- June 12, 2018
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain OP139 (wgIs139(ceh-27::TY1 EGFP 3xFLAG(P000007_F10); unc-119(+));unc-119(ed3)) mixed sex young adult (73 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans ceh-27 | ENCBS491SZL | ENCGM959ATT | ENCAB728YTO | ENCLB503SWZ |
| 4 | 1 | Caenorhabditis elegans strain OP139 (wgIs139(ceh-27::TY1 EGFP 3xFLAG(P000007_F10); unc-119(+));unc-119(ed3)) mixed sex young adult (73 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans ceh-27 | ENCBS119MIW | ENCGM959ATT | ENCAB728YTO | ENCLB772SGX |