Experiment summary for ENCSR949ZZD

Summary

Status
released
Assay
HiC (intact Hi-C)
Biosample summary
Homo sapiens T-helper 1 cell male adult (35 years) treated with 30 ng/mL Interleukin-12 subunit beta for 36 hours, 30 ng/mL Interleukin-12 subunit alpha for 36 hours, 1 μg/mL Interleukin-4 antibody for 36 hours
Biosample Type
primary cell
Replication type
unreplicated
Description
Naive CD4 Th1 Resting LP18 intact Mnase HiC
Treatments
30 ng/mL Interleukin-12 subunit beta (UniProtKB:P29460) for 36 hours, 30 ng/mL Interleukin-12 subunit alpha (UniProtKB:P29459) for 36 hours, 1 μg/mL Interleukin-4 antibody for 36 hours
Nucleic acid type
DNA
Library starting quantity
1000000cells
Size range
100-400
Fragmentation methods
chemical (micrococcal nuclease)
Size selection method
SPRI beads

Attribution

ENCODE4 project
Lab
Erez Aiden, Baylor
Award
UM1HG009375 (Erez Aiden, Baylor)
Project
ENCODE
Aliases
erez-aiden:naivecd4-th1-resting-intact
Date released
August 5, 2024

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Homo sapiens male adult (35 years) T-helper 1 primary cell treated with 30 ng/mL Interleukin-12 subunit beta for 36 hours, 30 ng/mL Interleukin-12 subunit alpha for 36 hours, 1 μg/mL Interleukin-4 antibody for 36 hoursENCBS853JPUENCLB589PQB
12Homo sapiens male adult (35 years) T-helper 1 primary cell treated with 30 ng/mL Interleukin-12 subunit beta for 36 hours, 30 ng/mL Interleukin-12 subunit alpha for 36 hours, 1 μg/mL Interleukin-4 antibody for 36 hoursENCBS853JPUENCLB260FYN