Experiment summary for ENCSR943LKA

Summary

Status
released
Assay
RNA-seq (polyA RNA-seq)
Biosample summary
Mus musculus C57BL/6 hindbrain embryo (10.5 days)
Biosample Type
tissue
Replication type
isogenic
Description
PolyA RNA-Seq from oligo-dT primed Total RNA on 10ng of embryonic 10.5 day mouse hindbrain
Nucleic acid type
polyadenylated mRNA
RNA integrity number
9.8 [1-1]10 [2-1]
Library starting quantity
10.0ng
Size range
>200
Lysis method
Ambion mirVana
Extraction method
Ambion mirVana
Fragmentation method
chemical (Nextera tagmentation)
Size selection method
SPRI beads
Strand specificity
Non-strand-specific
Spike-ins datasets
ENCSR884LPM

Attribution

ENCODE3 project
Lab
Barbara Wold, Caltech
Award
U54HG006998 (Richard Myers, HAIB)
Project
ENCODE
External resources
Date submitted
July 28, 2016
Date released
August 11, 2016
Related datasets
ENCSR050BJO 
Reference epigenome for mouse hindbrain (embryonic 10.5 day).
Tags
Search for all Experiment with internal tag ENCYCLOPEDIAv4

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleLibrary
11strain B6NCrl mixed sex embryo (10.5 days) hindbrain tissueENCBS626UIAENCLB554ZAM
21strain B6NCrl mixed sex embryo (10.5 days) hindbrain tissueENCBS723QMZENCLB838FEJ