Experiment summary for ENCSR925DZW
doi:10.17989/ENCSR925DZW
Summary
- Status
- released
- Assay
- RNA-seq (total RNA-seq)
- Biosample summary
- Homo sapiens activated T-cell male adult (43 years) treated with 50 U/mL Interleukin-2 for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours
- Biosample Type
- primary cell
- Replication type
- unreplicated
- Description
- CD3+_DONOR 1: 72 Hour T cell activation (CD3.CD28 beads) + 50U/ml IL-2
- Treatments
- 50 U/mL Interleukin-2 (UniProtKB:P60568) for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours
- Nucleic acid type
- RNA
- RNA integrity number
- 9 [1-2]10 [1-1]
- Library starting quantity
- 330ng
- Average fragment size
- 286 [1-2]282 [1-1]
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR597UQL
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE4 project
- Lab
- Barbara Wold, Caltech
- Award
- UM1HG009443 (Barbara Wold, Caltech)
- Project
- ENCODE
- External resources
- Date released
- July 1, 2022
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 2 | Homo sapiens male adult (43 years) activated T-cell primary cell treated with 50 U/mL Interleukin-2 for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours | ENCBS969BFR | ENCLB992WSI |
| 1 | 1 | Homo sapiens male adult (43 years) activated T-cell primary cell treated with 50 U/mL Interleukin-2 for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours | ENCBS969BFR | ENCLB796NXO |