Experiment summary for ENCSR919QDM
doi:10.17989/ENCSR919QDM
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- ets-4
- Biosample summary
- Caenorhabditis elegans strain RAF1713 (rrrSi400[Pets-4::ets-4-GFP::ets-4 3'UTR, unc-119(+)] II; unc-119(ed3) III) whole organism young adult (73 hours) at L1 larva starved after bleaching stage genetically modified (insertion) using site-specific recombination targeting C. elegans ets-4
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing ets-4-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:ETS-4_RAF1713_yAd
- Date submitted
- May 30, 2018
- Date released
- June 13, 2018
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
---|---|---|---|---|---|---|
1 | 1 | Caenorhabditis elegans strain RAF1713 (rrrSi400[Pets-4::ets-4-GFP::ets-4 3'UTR, unc-119(+)] II; unc-119(ed3) III) mixed sex young adult (73 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using site-specific recombination targeting C. elegans ets-4 | ENCBS272BWR | ENCGM819YKB | ENCAB728YTO | ENCLB273PWC |
2 | 1 | Caenorhabditis elegans strain RAF1713 (rrrSi400[Pets-4::ets-4-GFP::ets-4 3'UTR, unc-119(+)] II; unc-119(ed3) III) mixed sex young adult (73 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using site-specific recombination targeting C. elegans ets-4 | ENCBS040LZG | ENCGM819YKB | ENCAB728YTO | ENCLB372DCT |