Experiment summary for ENCSR919QDM

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain RAF1713 (rrrSi400[Pets-4::ets-4-GFP::ets-4 3'UTR, unc-119(+)] II; unc-119(ed3) III) whole organism young adult (73 hours) at L1 larva starved after bleaching stage genetically modified using site-specific recombination
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing ets-4-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:ETS-4_RAF1713_yAd
Date submitted
May 30, 2018
Date released
June 13, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
11strain RAF1713 (rrrSi400[Pets-4::ets-4-GFP::ets-4 3'UTR, unc-119(+)] II; unc-119(ed3) III) mixed sex young adult (73 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-ets-4ENCBS272BWRENCGM819YKBENCAB728YTOENCLB273PWC
21strain RAF1713 (rrrSi400[Pets-4::ets-4-GFP::ets-4 3'UTR, unc-119(+)] II; unc-119(ed3) III) mixed sex young adult (73 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-ets-4ENCBS040LZGENCGM819YKBENCAB728YTOENCLB372DCT