Experiment summary for ENCSR909XXF

Summary

Status
released
Assay
ChIP-seq
Target
eGFP-PHDP
Biosample summary
Drosophila melanogaster VK00033 whole organism embryo (10-24 hours) genetically modified using site-specific recombination
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing -eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:PHDP-GFP
Date submitted
September 18, 2018
Date released
September 26, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
21strain PHDP embryo (10-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-PHDPENCBS216HPBENCGM220DSKENCAB728YTOENCLB925ITI
11strain PHDP embryo (10-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-PHDPENCBS053LBGENCGM220DSKENCAB728YTOENCLB594URO
31strain PHDP embryo (10-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-PHDPENCBS744RBTENCGM220DSKENCAB728YTOENCLB044PDE