Experiment summary for ENCSR902BCW
Summary
- Status
- released
- Assay
- HiC (intact Hi-C)
- Biosample summary
- Homo sapiens activated T-helper 1 cell male adult (35 years) treated with anti-CD3 and anti-CD28 coated beads for 24 hours, 30 ng/mL Interleukin-12 subunit beta for 36 hours, 10 ng/mL Interleukin-2 for 14 days, anti-CD3 and anti-CD28 coated beads for 14 days, 1 μg/mL Interleukin-4 antibody for 36 hours, 30 ng/mL Interleukin-12 subunit alpha for 36 hours
- Biosample Type
- primary cell
- Replication type
- unreplicated
- Description
- Naive CD4 Th1 activated LP18 intact Mnase HiC
- Treatments
- anti-CD3 and anti-CD28 coated beads for 24 hours, 30 ng/mL Interleukin-12 subunit beta (UniProtKB:P29460) for 36 hours, 10 ng/mL Interleukin-2 (UniProtKB:P60568) for 14 days, anti-CD3 and anti-CD28 coated beads for 14 days, 1 μg/mL Interleukin-4 antibody for 36 hours, 30 ng/mL Interleukin-12 subunit alpha (UniProtKB:P29459) for 36 hours
- Nucleic acid type
- DNA
- Library starting quantity
- 1000000cells
- Size range
- 100-400
- Fragmentation methods
- chemical (micrococcal nuclease)
- Size selection method
- SPRI beads
- Platform
- Ultima Genomics
Attribution
ENCODE4 project
- Lab
- Erez Aiden, Baylor
- Award
- UM1HG009375 (Erez Aiden, Baylor)
- Project
- ENCODE
- Aliases
- erez-aiden:naivecd4-th1-activated-intact
- Date released
- August 5, 2024
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Homo sapiens male adult (35 years) activated T-helper 1 primary cell treated with anti-CD3 and anti-CD28 coated beads for 24 hours, 30 ng/mL Interleukin-12 subunit beta for 36 hours, 10 ng/mL Interleukin-2 for 14 days, anti-CD3 and anti-CD28 coated beads for 14 days, 1 μg/mL Interleukin-4 antibody for 36 hours, 30 ng/mL Interleukin-12 subunit alpha for 36 hours | ENCBS951MKM | ENCLB042CSN |
| 1 | 2 | Homo sapiens male adult (35 years) activated T-helper 1 primary cell treated with anti-CD3 and anti-CD28 coated beads for 24 hours, 30 ng/mL Interleukin-12 subunit beta for 36 hours, 10 ng/mL Interleukin-2 for 14 days, anti-CD3 and anti-CD28 coated beads for 14 days, 1 μg/mL Interleukin-4 antibody for 36 hours, 30 ng/mL Interleukin-12 subunit alpha for 36 hours | ENCBS951MKM | ENCLB140EJP |