Experiment summary for ENCSR889TRN
doi:10.17989/ENCSR889TRN
Supersedes ENCSR000AJD
Summary
- Status
- released
- Assay
- polyA plus RNA-seq
- Biosample summary
- Homo sapiens GM12878
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- PolyA RNA-Seq from oligo-dT primed Total RNA on bulk prep (10 pg) from the GM12878 cell line
- Nucleic acid type
- polyadenylated mRNA
- Library starting quantity
- 10ng
- Size range
- >200
- Extraction method
- Ambion mirVana
- Fragmentation methods
- chemical (Nextera tagmentation)
- Size selection method
- SPRI beads
- Strand specificity
- unstranded
- Spike-ins datasets
- ENCSR884LPM
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Barbara Wold, Caltech
- Award
- U54HG006998 (Richard Myers, HAIB)
- Project
- ENCODE
- External resources
- Aliases
- barbara-wold:single-cell-13274-exp
- Date submitted
- July 28, 2016
- Date released
- June 13, 2016
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
1 | 1 | Homo sapiens GM12878 cell line | ENCBS195IGI | ENCLB360EJW |
2 | 1 | Homo sapiens GM12878 cell line | ENCBS195IGI | ENCLB855HEY |