Experiment summary for ENCSR889PUP

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain OP656 (unc-119 (tm4063) III; wgIs656) whole organism early embryo 45 minutes post synchronization using egg bleaching genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing HMBX-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation method
sonication (generic microtip)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:HMBX-1-OP656-EE
Date submitted
October 4, 2016
Date released
October 11, 2016
Submitter comment
ENCFF357GIB, ENCFF092EEF, ENCFF457JXB, ENCFF809OBJ were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
11strain OP656 (unc-119 (tm4063) III; wgIs656) mixed sex early embryo 45 minutes post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-hmbx-1ENCBS015MDHENCGM212KJVENCAB728YTOENCLB818AUP
21strain OP656 (unc-119 (tm4063) III; wgIs656) mixed sex early embryo 45 minutes post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-hmbx-1ENCBS188TXXENCGM212KJVENCAB728YTOENCLB545YKR