Experiment summary for ENCSR889PUP

doi:10.17989/ENCSR889PUP

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
hmbx-1
Biosample summary
Caenorhabditis elegans strain OP656 (unc-119 (tm4063) III; wgIs656) whole organism early embryo 45 minutes post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans hmbx-1
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing HMBX-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:HMBX-1-OP656-EE
Date submitted
October 4, 2016
Date released
October 11, 2016
Submitter comment
ENCFF357GIB, ENCFF092EEF, ENCFF457JXB, ENCFF809OBJ were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Caenorhabditis elegans strain OP656 (unc-119 (tm4063) III; wgIs656) mixed sex early embryo 45 minutes post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans hmbx-1ENCBS015MDHENCGM212KJVENCAB728YTOENCLB818AUP
21Caenorhabditis elegans strain OP656 (unc-119 (tm4063) III; wgIs656) mixed sex early embryo 45 minutes post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans hmbx-1ENCBS188TXXENCGM212KJVENCAB728YTOENCLB545YKR